Gold nanoparticles enhanced electrochemical impedance sensor for human papillomavirus (HPV) 16 detection using synthetic target

Abstract

The Human Papillomavirus (HPV) is the most common sexually transmitted virus. Worldwide most common high risk HPV 16 and approximately 70% of cervical cancers are due to infection by these genotypes. The persistent infection by high risk HPV is a necessary but not sufficient cause of this cancer which develops over a long period through precursor lesions which can be detected by electrochemical impedance sensor. Although this method shown decreased the incidence of cervical cancer, HPV related cervical disease. The HPV driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of women with HPV related cervical disease and these biomarkers can also be used to increase the positive predictive value of current methods. The most influential methods for the detection and identification of HPV using gold nanoparticle (GNP) included electrochemical impedance sensor will visit their sensitivity, selectivity and characteristic detection on synthetic target which are complement of the DNA, non-complement of the DNA and mismatch of the DNA. In difference concentration of synthetic target, which stage can get the exactly value to determine the HPV in strain 16 was evaluated in this thesis.