| dc.description.abstract | This project was aim on evaluation of the antibacterial and antioxidation activities
of plant extracts by concerning the antibiotic resistance problem. Stachytarpheta indica(L.) Vahl is a traditional medical plant used in Malaysia as anti-inflammatory and painrelief
agents. In this project, the root, stem and leave extracts from S. indica (L.) Vahl
were obtained through aqueous extraction (maceration, decoction, autoclave) and organic
solvent extraction (soxhlet multiply extraction using pet-ether, chloroform, and ethanol
successively and exhaustively).The yield of S. indica (L.) Vahl extracts from different
extraction methods was calculated and interpreted. Bacterial susceptibility test was
performed using disk diffusion method on two gram-positive bacteria, Streptococcus
aureus, Bacillus subtilis and one gram-negative bacteria, Salmonella typhi, but results
shown that S. indica (L.) Vahl extracts do no exhibit antibacterial activity. The total
phenolic content and antioxidant activity of extracts were evaluated through
Folin-Ciocalteu assay and 2, 2-diphenyl-2-picryl-hydrazyl (DPPH) assay. From the
results, the S. indica (L.) Vahl extracts were shown to contain satisfied phenolic content
ranged from 29.195 ± 0.520 to 74.475 ± 0.900 mg GAE/g and possessed antioxidant
activity ranged from 5.524% ± 1.725 to 42.639% ± 1.370 which may, in part, be
associated with phenolic compounds. Phytochemical analysis of the S. indica (L.) Vahl
extracts was done using preliminary phytochemical tests, the results revealed the presence
of tannin, flavonoid and phenol which may have contribute to antioxidant activity. | en_US |
