| dc.description.abstract | Nowadays, overuse of chemical fungicide has side effect which is harmful to human
consumption, causes environmental problem, and destroy the antagonist microorganisms. Overuse of antibiotic also resulted to multi drug-resistant (MDR), where antibiotics become ineffective against these bacterial strains. Therefore, it is
important to develop natural fungicide and bactericide from plant extract. For the
current study, chilli anthracnose fungus, Colletotrichumcapsici and bacteria Bacillus
subtilis were used for antifungal and antibacterial activity. C. capcisi was isolated
from chilli and its morphology was identified by its white colony colour, fluffy
cottony look on PDA, rings of acervuli and curved conidia. Antifungal activity was
demonstrated by using different concentration C. odollam flower extract (500 ppm to
4000 ppm). C. odollam has low inhibition against C. capsici at 3000 ppm and 4000
ppm. As for the antibacterial activity, different concentrations of C. odollam flower
extracts (1.25 mg/ml until 200 mg/ml) were tested on B. subtilis by using disk
diffusion method. C. odollam has moderate inhibition against B. subtilis (11.30
mg/ml).B. subtilis proved that MIC value is 5 mg/ml. The phytochemical test
confirmed the presence of steroids, glycosides, triterpenoids, terpenoids, tannin and
phenolic compound. In conclusion, C. odollam is not suitable to replace chemical
fungicide against C. capsici. On the other hand, C. odollam is suitable to be
commercialized as natural bactericide. | en_US |
