Production of glucose from sugarcane bagasse for bioethanol application: optimization studies

Abstract

Sugarcane Bagasse (SCB) is one example of lignocellulosic biomass and usually discarded as industrial waste after sugarcane is crushed to obtain its juices. It consists of cellulose (42%), hemicellulose (25%), and lignin (20%). There are mainly three processing method involved in the glucose production which is pretreatment, enzymatic hydrolysis, and fermentation. The pretreatment were carried out to remove lignin and hemicellulose to enhance the enzymatic hydrolysis process. Through enzymatic hydrolysis, glucose were produced from sugarcane bagasse. In this research, Response Surface Methodology (RSM) were used to evalute the interactive effects of enzymatic hydrolysis of substrate concentration (2 – 10 % (w/v)), enzyme concentration (10 – 15 % (v/w)), and time (3 – 8 hrs). The optimum condition obtained through RSM were substrate concentration (10 % (w/v)), enzyme concentration (10 % (v/w)), and time (3 hrs). The yield glucose concentration was found to be 0.033 g/L under optimum condition. A model adequacy was very satisfactory, as coefficient of determination were 0.9308 (93.08%). Fermentation with S.cerevisiae were carried out to produce bioethanol and oxidation test were used to verify the presence of bioethanol.